DESCRIPTION MW= 38.8 kDa. Full length nsp15 (endoRNAse) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-346 (NCBI reference sequence YP_009725310.1).
SEQUENCE Numeration of the nsp15 from NCBI YP_009725310.1
10 20 30 40 50 60 SLENVAFNVV NKGHFDGQQG EVPVSIINNT VYTKVDGVDV ELFENKTTLP VNVAFELWAK 70 80 90 100 110 120 RNIKPVPEVK ILNNLGVDIA ANTVIWDYKR DAPAHISTIG VCSMTDIAKK PTETICAPLT 130 140 150 160 170 180 VFFDGRVDGQ VDLFRNARNG VLITEGSVKG LQPSVGPKQA SLNGVTLIGE AVKTQFNYYK 190 200 210 220 230 240 KVDGVVQQLP ETYFTQSRNL QEFKPRSQME IDFLELAMDE FIERYKLEGY AFEHIVYGDF 250 260 270 280 290 300 SHSQLGGLHL LIGLAKRFKE SPFELEDFIP MDSTVKNYFI TDAQTGSSKC VCSVIDLLLD 310 320 330 340 DFVEIIKSQD LSVVSKVVKV TIDYTEISFM LWCKDGHVET FYPKLQ
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 33140 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.